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KMID : 0379120100380010034
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Korean Journal of Mycology
2010 Volume.38 No. 1 p.34 ~ p.39
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Molecular Genetic Classification of Hypsizigus marmoreus and Development of Strain-specific DNA Markers
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Lim Yun-Jeong
Lee Chang-Yun
Park Jeong-Eun
Kim Sang-Woo
Lee Hyun-Sook
No Hyun-Soo
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Abstract
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We have attempted to verify 30 strains of Hypsizigus marmoreus from various mushroom stocks in Korea using random amplified polymorphic DNA (RAPD) methodology. Chromosomal DNAs of them were extracted and subjected to PCR analyses with 3 random primers. Each PCR produced approximately 30 distinct PCR bands with the size from 200 bp to 3000 bp. A dendrogram was acquired using the unweighted pair-group method with arithmetic average (UPGMA) clustering methodology on the basis of the DNA band pattern. The analysis revealed that 30 strains of H. marmoreus were clustered into two distinct clusters. Cluster 1 contained 3 subgroups while the cluster 2 consisted of rather diverse strains. Interestingly, Hm3-10, a wild strain collected from Deog-Yu mountain, was not included in either clusters, indicative of uniqueness of this strain. We nextly attempted to develop strain-specific DNA markers to verify a specific strain. A unique band in the RAPD gel lane of Hm0-4 was extracted and its sequence was determined. PCR with a primer set from the determined sequence revealed that the primer set gave a 250 bp DNA band only for Hm0-4, indicating that this approach works well for the strain-specific identification of H. marmoreus.
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KEYWORD
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Classification, DNA marker, Hypsizigus marmoreus, RAPD
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